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DETERMINATION OF THE VERTICAL TRASMISSION OF DUCK ENTERITIS VIRUS FROM DUCK BREEDERS TO DUCKLINGS BY USING PCR AND RT- PCR TECHNIQUES. Update: 9/20/2012 4:14:33 PM (GMT+7)

Tran Ngoc Bich and Jean-Luc GUERIN - To confirm the vertical transmission of Duck Enteritis Virus (DEV) from duck breeders to their ducklings, we have used methods of molecular biology tests (PCR and RT-PCR). The result of investigation was confirmed to have the vertical transmission of DEV from infected duck breeders to their ducklings.

SUMMARY

To confirm the vertical transmission of Duck Enteritis Virus (DEV) from duck breeders to their ducklings, we have used methods of molecular biology tests (PCR and RT-PCR). The result of investigation was confirmed to have the vertical transmission of DEV from infected duck breeders to their ducklings. This result was verified through clinical symptoms, disease characteristics and PCR tests (Hansen , 2000).  148 samples of one day old ducklings and dead embryos collecting from DEV infected Peking duck reproduction farm were tested by PCR techniques. Testing results indicated that DEV infected ratio varied from 0 to 4.6 % by classical PCR technique, and from 79 to 97.3%  by PCR-RT technique, with marking substance - SYBR Green  Master Mix (Applied Bio Systems).

        Key words: Duck, DEV,Transmission, PCR, RT-PCR

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