vị trí 0
1
1
English | VietNamse
Today, Sunday, 23/07/2017
Menu
Exchange
Loại
Buy
Sell

SJC

Quảng cáo trái vị trí 1
Quảng cáo trái vị trí 2
Hoi nghị
Quảng cáo trái vị trí 3
Quảng cáo trái vị trí 4
Quảng cáo trái vị trí 5
Quảng cáo trái vị trí 6
Quảng cáo trái vị trí 7
Quảng cáo trái vị trí 8
Quảng cáo trái vị trí 9
Quảng cáo trái vị trí 10
Submit Thread to Facebook Submit Thread to Twitter Submit Thread to Google Email Print
Study on the pathogenicity and immune response of Nervous Necrosis Virus (NNV) in grouper culture in Vietnam Update: 9/20/2012 4:35:46 PM (GMT+7)

Pham Thi Tam, Pham Cong Hoat, Nguyen Thi Thu Hien, Vu Tien Lam, Nguyen Thi Vui and Nguyen Thi Thanh - Eight strains of Nervous Necrosis Virut (NNV) were used to study on pathogenecity and inducing immune response in grouper being cultured in Vietnam. These virus strains having different virulences obtained through assessment of pathogenous levels in susceptible cells and grouper fry/fingerlings, with index TCID50 reached from 10-2,7 to 10-3,9, index LD50 reached from 10-1,5 to 10-7,5. Of which, four NNV strains namely QN 02, QN 05, QN 07, KH 05 were

Summary

Eight strains of Nervous Necrosis Virut (NNV) were used to study on pathogenecity and inducing immune response in grouper being cultured in Vietnam. These virus strains having different virulences obtained through assessment of pathogenous levels in susceptible cells and grouper fry/fingerlings, with index TCID50 reached from 10-2,7 to 10-3,9, index LD50 reached from 10-1,5 to 10-7,5. Of which, four NNV strains namely QN 02, QN 05, QN 07, KH 05 were determined to have high virulence, causing 100 % groupers died after 3 post infection. Evaluation of inducing immune response of these virus strains was determined that KH 05 virus strain was highest antigen representative. This virus strain was inactivated by formalin 0.3 %, NNV KH 05 was emulsified by Montanide ISA 70 that could generate antibody, neutralized with high virulence NNV at day 15th in rabbits and antibody protected 80 – 85 % in groupers at day 20th after inducing immunity. This result could be used as basis for producing vaccine against VNN  disease.     

Key words: Grouper, Nervous Necrosis Virus (NNV), TCID50, LD50,  Neutralizing antibody, Protective antibody

News
Focus
Entertainment
Statistics
Online : 70
Totals : 5,431,021
VIETNAM VETERINARY ASSOCIATION
Address: 86 Truong Chinh, Dong Da, Ha Noi - Tel: (04) 3869 1082

Copyright © 2012 Vietnam Veterinary Medical Association - Designed and developed by Bambu®